Wageningen University is an internationally leading education and research organization and is with more than 5,000 employees the largest organization in the Netherlands focusing on life sciences. Wageningen University participates in the project via the Laboratory of Microbiology (MIB), which groups 6 professors and approximately 100 other scientists, PhD and MSc students, and supporting staff. MIB aims to understand microbial interactions, biotransformations and their control in bacteria, archaea and fungi. MIB research is characterized by a focus on molecular mechanisms, genomics-driven approaches, and applications in food, non-food and environmental biotechnology. It has contributed to the first published crenarchaeotic genome (Sulfolobus solfataricus) the first genome of lactobacilli (Lactobacillus plantarum) and various other genome sequences of novel intestinal and anaerobic isolates. It has pioneered the first molecular methods to determine the human intestinal microbiota. MIB has within TI Food and Nutrition determined the in situ response of L. plantarum in the human intestine and its interactions with the host. Currently, it is engaged in the functional metagenomics analysis of the human ileum microbiota. Within MetaHIT, MIB will coordinate the activities in the sequence based functionality workpackage, its tasks include sequence mining and functional genomic approaches to elucidate microbiota gene function, focusing on host interactions, and exploiting efficient gene expression tools developed by its scientists. In addition, the sequencing pillar will employ the high throughput microbial diversity diagnostics array, Human Intestinal Tract (HIT) Chip, developed by MIB.